peptides

CJC-1295: GHRH Analog Research Guide

Complete research guide for CJC-1295, a modified growth hormone-releasing hormone (GHRH) analog with extended activity.

8 min readUpdated Jan 2025

CJC-1295: Research Overview

CJC-1295 is a synthetic analog of growth hormone-releasing hormone (GHRH) with significant modifications to extend its biological activity. It comes in two primary forms: CJC-1295 with DAC (Drug Affinity Complex) and CJC-1295 without DAC (also called Modified GRF 1-29).

Peptide Variants

CJC-1295 with DAC

PropertyDetails
TypeGHRH analog + DAC
Amino Acids30
DACLysine linked to maleimidopropionic acid
Half-lifeExtended (days)
Molecular Weight~3647 g/mol

CJC-1295 without DAC (MOD GRF 1-29)

PropertyDetails
TypeModified GHRH (1-29)
Amino Acids29
DACNone
Half-lifeShort (minutes)
Molecular Weight~3367 g/mol

Understanding GHRH

Natural GHRH

Growth hormone-releasing hormone:

  • 44 amino acids in full form
  • Released from hypothalamus
  • Stimulates pituitary GH release
  • Rapidly degraded by DPP-IV enzyme
  • The Challenge

    Natural GHRH limitations:

  • Very short half-life (minutes)
  • Rapid enzymatic degradation
  • Impractical for research applications
  • Led to development of modified analogs
  • CJC-1295 Modifications

    Amino Acid Substitutions

    Key modifications for stability:

  • Position 2: Ala → D-Ala (DPP-IV resistance)
  • Position 8: Gln → Asn
  • Position 15: Gly → Ala
  • Position 27: Met → Leu (oxidation resistance)
  • The DAC Technology

    Drug Affinity Complex (DAC):

  • Allows binding to serum albumin
  • Dramatically extends half-life
  • Creates sustained activity profile
  • Unique to "with DAC" version
  • Research Applications

    GH Axis Research

  • GHRH receptor studies
  • GH pulsatility research
  • IGF-1 pathway studies
  • Somatotroph function
  • Comparative Studies

    ComparisonResearch Focus
    With DAC vs. without DACDuration of action
    CJC-1295 vs. natural GHRHStability/potency
    CJC-1295 + GHRPSynergistic effects

    Combination Research

    Often studied with:

  • Ipamorelin (popular combination)
  • GHRP-6
  • GHRP-2
  • Hexarelin
  • Reconstitution Protocol

    Standard Volumes

    VariantVial SizeBAC WaterConcentration
    With DAC2mg2ml1,000mcg/ml
    Without DAC2mg2ml1,000mcg/ml
    Without DAC5mg2.5ml2,000mcg/ml

    Procedure

  • Allow vial to reach room temperature
  • Clean stopper with alcohol swab
  • Add BAC water slowly along vial wall
  • Let dissolve naturally
  • Do not shake
  • Verify clear solution
  • Storage Guidelines

    Lyophilized

  • Freezer (-20°C): 2+ years
  • Refrigerator (2-8°C): 6-12 months
  • Reconstituted

  • Temperature: 2-8°C only
  • Duration: 4-6 weeks
  • Never freeze
  • Choosing Between Variants

    CJC-1295 with DAC

    Best for:

  • Sustained activity studies
  • Less frequent dosing protocols
  • Steady-state research
  • CJC-1295 without DAC (MOD GRF)

    Best for:

  • Pulsatile release studies
  • Combination with GHRP
  • Natural rhythm mimicry
  • Acute response research
  • Physical Properties

    Lyophilized:

  • White to off-white powder
  • May appear as cake
  • Reconstituted:

  • Clear, colorless solution
  • No particles
  • Quality Indicators

    Acceptable

  • Clear solution
  • Complete dissolution
  • No color change
  • Proper storage maintained
  • Problems

  • Cloudiness
  • Particles
  • Discoloration
  • Slow dissolution
  • Conclusion

    CJC-1295 provides researchers with stable GHRH analogs for studying the growth hormone axis. The choice between DAC and non-DAC versions depends on whether sustained or pulsatile activity is desired for specific research applications.

    *CJC-1295 is sold for research purposes only and is not approved for human use.*

    Frequently Asked Questions

    What is the difference between CJC-1295 with DAC and without DAC?

    CJC-1295 with DAC has a Drug Affinity Complex that allows albumin binding, creating extended activity lasting days. Without DAC (MOD GRF 1-29), activity is much shorter (minutes), allowing for pulsatile GH release studies.

    Why is CJC-1295 often combined with Ipamorelin?

    CJC-1295 (GHRH analog) and Ipamorelin (GHRP) work through different receptors that synergize for GH release. The combination is studied to examine additive or synergistic effects on the GH axis.

    What modifications make CJC-1295 more stable than natural GHRH?

    CJC-1295 has amino acid substitutions including D-alanine at position 2 (DPP-IV resistance) and leucine replacing methionine at position 27 (oxidation resistance). These changes dramatically improve stability.

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